The aim of this study was to characterize oils from black cumin and lupine obtained by supercritical CO2 extraction using ethanol as a co-solvent. SFE extraction process conditions were as follows: temperature 50°C and pressure 300 bars. The oils were characterized by evaluating the fatty acid profile, and the content of tocochromanols and phytosterols. The main fatty acids in oil from black cumin were linoleic acid (57.69%) and oleic acid (24.84%). The main fatty acids identified in lupin oil were oleic acid (59.84%), linoleic acid (15.6%), and linolenic acid (13.39%). The oils also differed in the content and composition of tocochromanols. In oil from black cumin and lupin, the content of tocochromanols was 28.97 mg/100 g of oil and 189.49 mg/100 g of oil, respectively. In oil from black cumin, α-, γ-, and ∂-tocopherol and α-, β- γ-, and ∂-tocotrienols were present. In lupin oil, only tocopherols and PS-8 were present. In oil from black cumin, the main tocochromanols were β-tocotrienol (16.18 mg/100 g oil) and α-tocopherol (9.64 mg/100 g oil). In lupin oil, γ-tocopherol (178.67 mg/100 g oil) was identified in the highest amount. Significant differences were also found in the amount and composition of phytosterols. Their total content was 219.41 and 1917.86 mg/100 g of oil in black cumin oil and lupine oil, respectively. In oil from black cumin, the main sterols were ß-sitosterol (73.57 mg/100 g oil) and cycloartenol (42.40 mg/100 g oil). In lupin oil, the sterols with the highest amount were lupeol (837.03 mg/100 g oil) and ß-sitosterol (672.36 mg/100 g oil).
The research was financed by the UMO-2019/35/N/NZ9/00767 project from the funds of the National Science Centre.