OXI-006: Phospholipid association colloids facilitate synergistic interactions between ascorbyl palmitate and the antioxidants α-tocopherol and Trolox

It is widely accepted that reverse micelles, spontaneously formed by amphiphilic minor oil components, play a significant role in lipid oxidation in bulk oils by acting as microreactors that catalyze oxidative reactions [1]. However, their effect on antioxidant efficacy remains less well understood.

Recent studies have shown that egg yolk phosphatidylcholine reverse micelles (EYPC-RM) did not affect the antioxidant activity of α-tocopherol in stripped sunflower oil at 50 °C, but substantially reduced the efficacy of Trolox under the same experimental conditions [2]. Similarly, EYPC-RM also diminished the efficacy of other polar antioxidants, such as gallic acid and its ester derivatives, propyl and dodecyl gallates, suggesting that polar antioxidants are more strongly affected due to greater interaction with these preformed colloids.

In the present study, we explored the effect of reverse micelles on the synergistic interactions between ascorbyl palmitate (AP) and the antioxidants αtocopherol and Trolox, taken as representatives of nonpolar and polar antioxidants, respectively. Oxidative stability index (OSI) measurements at 100 °C showed that EYPC-RM enhanced the antioxidant efficacy of both the αtocopherol–AP and Trolox–AP pairs in stripped sunflower oil. While the efficacy of Trolox was substantially reduced in the presence of EYPC-RM, this reduction was not observed when Trolox was combined with AP, suggesting effective interactions between the two antioxidants at the reverse micelle interface. Furthermore, EPR analysis of samples incubated at 50 °C showed reduced radical activity in the presence of AP; however, when EYPC-RM was also present, increased radical formation was observed, suggesting partial loss of efficacy due to the prooxidative effect of reverse micelles.

Euro Fed Lipid e.V.
Data collection on our website

The Internet pages partly use so-called cookies. Cookies do not damage your computer and do not contain viruses. Cookies serve to make our offer more user-friendly, effective and safer. Cookies are small text files that are stored on your computer and saved by your browser.

Most of the cookies we use are so-called "session cookies". They are automatically deleted at the end of your visit. Other cookies remain stored on your computer until you delete them. These cookies enable us to recognize your browser the next time you visit us.

You can set your browser in such a way that you are informed about the setting of cookies and allow cookies only in individual cases, exclude the acceptance of cookies for certain cases or in general and activate the automatic deletion of cookies when closing the browser. If cookies are deactivated, the functionality of this website may be limited.

Cookies that are required to carry out the electronic communication process or to provide certain functions you have requested (e.g. shopping basket function) are stored on the basis of Art. 6 Para. 1 lit. f DSGVO. The website operator has a legitimate interest in the storage of cookies for the technically error-free and optimized provision of his services. Insofar as other cookies (e.g. cookies to analyze your surfing behavior) are stored, these are treated separately in this data protection declaration.